TLS 15A1 is a versatile synchrotron beamline designed to accommodate both academic and industrial users in 2013. This fully automated facility supports remote access, fostering international collaborations among research groups. TLS 15A1 offers a wide spectrum of applications, including high-throughput crystal screening, high-resolution data collection, de novo structure determination, and standard protein crystallography experiments. The energy range spans 5.6 keV to 15.5 keV (wavelengths 2.21 to 0.799 Å), with a flux of about 1.4×10¹¹ photons/s at the sample position through a 200 × 200 μm² aperture.

The beamline features a superconducting wiggler insertion device (In-Acromat, IASW6) as its light source, ensuring exceptional X-ray quality. X-rays are precisely collimated using a cylindrical collimating mirror and monochromated with a Si(111) double-crystal monochromator, followed by focused with a toroidal focusing mirror.

The end station is equipped with a cryo-cooler, a CCD X-ray detector (Rayonix MX300HE) and a micro-diffractometer (MD2). A high-speed air-bearing goniometer supports efficient crystal screening and phasing experiments, while an automatic sample changer (SAM system) enhances automation, enabling operation without human intervention. Remote access facilitates international collaborations in crystallography research.

• Cheng-Hung Chiang, et al., Biopharmaceutical Beamline TLS 15A1 for Macromolecular Crystallography at the National Synchrotron Radiation Research Center. J. Chin. Chem. Soc. (2024) 71, [doi: 10.1002/jccs.202400111]

• TLS 15A1 - Native Data Collection Methods_Chinese
• TLS 15A1 - MAD Data Collection Methods_Chinese
• Tutorial of Data Processing by HKL2000_Chinese
• Tutorial of Data Processing by HKL2000_English