The core technique of our facility is Synchrotron Radiation Protein Crystallography, a technique that uses the principle of single crystal X-ray diffraction to determine high-resolution three-dimensional protein molecular structure from a protein crystal. It is the most powerful and important method to study biological structures. Currently, nine out of every ten biological structures are solved by this technique. Worldwide, only 18 countries and the European Union possess synchrotron radiation facilities and provide protein crystallography technique, and Taiwan is included. Being the only facility in Taiwan that provides this technique, our goal is to build a world-class protein crystallographic facility to assist industrial and academic scientists and experts in the field of biomedical science (including structural biology, proteomics, biochemistry, molecular biology, drug design, biotechnology, etc.) to develop prevention, diagnosis, and treatment of various diseases. That helps improve the health of people, increase the quality of life and effectively conserve medical resources.
NSRRC PX Beamline |
TPS 07A Micro-focus Protein Crystallography |
TPS 05A Protein Microcrystallography |
TLS 15A1 Biopharmaceutical Protein Crystallography |
---|---|---|---|
X-ray source | TPS/ 3 GeV/ Undulator | TPS/ 3 GeV/ Undulator | TLS/ 1.5 GeV/ Wiggler |
General user time | 70% | 75% | 80% |
Status | Operational | Operational | Operational |
Experiment | MAD/Micro-focus beam | MAD/Microbeam | MAD |
Energy range (keV) | 6.0-20.0 | 5.7-20.0 | 5.6-15.5 |
Wavelength (Å) | 2.06-0.62 | 2.18-0.62 | 2.21-0.80 |
Aperture Size (µm) (types)* | 100-2 | 200-10 (9)* | 200-50 (5)* |
Flux After Aperture (p/s) | 820-86×1010 | 570-4.0×1010 | 14/8.5/3.7/2.2/1.0×1010 |
Flux Density (phots/s/µm2) | 9.4-1600×108 | 1.8-5.0×108 | 5.7-7.5×106 |
Time to Henderson Limit (s) | 0.24-42.6 | 12.1-15.4 | 5333-7018 |
Detector | EIGER2 X 16M | EIGER2 X 9M | MX300HE |
Frame Rate (frames/s) | 130 Hz | 230 Hz | 1 |
Sample Changer | ISARA | ISARA | SAM |
Remote Access | YES | YES | YES |
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- Eric Y C Mao, Han-Yi Yen, Chyuan-Chuan Wu, Structural basis of how MGME1 processes DNA 5' ends to maintain mitochondrial genome integrity. Nucleic Acids Research. 2024, 52, 4067. [doi: 10.1093/nar/gkae186]
- Chen-Hsi Chu, Che-Ting Wu,et al. , Insights into the molecular mechanism of ParABS system in chromosome partition by HpParA and HpParB. Nucleic Acids Research. 2024, 1. [doi: 10.1093/nar/gkae450]
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- Yuan, L., Ma, X., Yang, Y. et al. Phosphoantigens glue butyrophilin 3A1 and 2A1 to activate Vγ9Vδ2 T cells. Nature. 2023, 621, 840. [doi: 10.1038/s41586-023-06525-3]
- Huang, KY.A., Chen, X., Mohapatra, A. et al. Structural basis for a conserved neutralization epitope on the receptor-binding domain of SARS-CoV-2. Nat Commun. 2023,14 ,311. [doi: 10.1038/s41467-023-35949-8]
- Chen, NC., Wang, CH., Yoshimura, M. et al. Structures of honeybee-infecting Lake Sinai virus reveal domain functions and capsid assembly with dynamic motions. Nat Commun. 2023, 14, 545. [doi: 10.1038/s41467-023-36235-3]
- Nguyen, H.T.V., Chen, X., Parada, C. et al. Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome. Nat Commun. 2023, 14, 1903. [doi: 10.1038/s41467-023-37543-4]
- Wang, YL., Chang, CY., Hsu, NS. et al. N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry. Nat Commun. 2023, 14, 2528. [doi: 10.1038/s41467-023-38218-w]